Preprint / Version 1

HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts

Authors

  • Piyanee Ratanachamnong aDepartment of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
  • Yotsayut Chunchaowarit bDepartment of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand
  • Poommaree Namchaiw cBiological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok, Thailand
  • Cholticha Niwaspragrit eExpert Center of Innovative Agriculture, Thailand Institute of Scientific and Technological Research, Pathum Thani, Thailand
  • Punyabhorn Rattanacheeworn bDepartment of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand
  • Yamaratee Jaisin bDepartment of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand

Keywords:

C. hystrix water Extract, Bioactive compounds, Antioxidant, Cell migratory activity

Abstract

Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine for health benefits including oral and gum health, stress relief, hair care, and skincare. In this study, we demonstrated the antioxidant activity of C. hystrix water extract and its effect on human keratinocyte and fibroblast migration. The extract showed a high amount of phenolic and flavonoid contents. The HPLC analysis indicated the presence of gallic acid, catechin, caffeic acid, rutin, and quercetin. We showed that C. hystrix water extract exhibited free radical scavenging capacity, determined by DPPH assay, with IC50 of 14.91 mg/mL, and nitrite radical scavenging capacity, determined by NO assay, with IC50 of 4.46 mg/mL. The C. hystrix water extract displayed unnoticeable toxicity at all tested doses. We showed that the treatment of water extracts as low as 50 μg/mL decreased the reactive oxygen species (ROS) from H2O2-induced ROS formation in both cell lines. Besides, C. hystrix water extract promoted cell migration in a dose-dependent manner. Together, these results demonstrated the positive benefit of C. hystrix water extract as a wound-healing accelerator. Its health benefits may be due to the antioxidant capability of its phytochemical compounds contained in C. hystrix water extract that enhances the migration of two major cell types: fibroblast and keratinocytes, responsible for the proliferation and remodeling phase of wound healing. Keywords: C. hystrix water Extract, Bioactive compounds, Antioxidant, Cell migratory activity

Author Biography

Poommaree Namchaiw, cBiological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok, Thailand

dNeuroscience Center for Research and Innovation, Learning Institute, King Mongkut's University of Technology Thonburi, Bangkok, Thailand