Preprint / Version 1

Metabolomics-Based Profiling via a Chemometric Approach to Investigate the Antidiabetic Property of Different Parts and Origins of Pistacia lentiscus L.

Authors

  • Chabha Sehaki BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France
  • Roland Molinie BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France
  • David Mathiron Analytical Platform, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France
  • Jean-Xavier Fontaine BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France
  • Nathalie Jullian BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France
  • Fadila Ayati Laboratory of Natural Resources, University Mouloud Mammeri of Tizi-Ouzou, Tizi-Ouzou 15000, Algeria
  • Farida Fernane Laboratory of Natural Resources, University Mouloud Mammeri of Tizi-Ouzou, Tizi-Ouzou 15000, Algeria
  • Eric Gontier BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Keywords:

P. lentiscus, α-glucosidase, phytochemical profiling, UHPLC-ESI-HRMS, metabolomic approach, antidiabetic

Abstract

Pistacia lentiscus L. is a medicinal plant that grows spontaneously throughout the Mediterranean basin and is traditionally used to treat diseases, including diabetes. The aim of this work consists of the evaluation of the α-glucosidase inhibitory effect (i.e., antidiabetic activity in vitro) of different extracts from the leaves, stem barks and fruits of P. lentiscus harvested on mountains and the littoral of Tizi-Ouzou in Algeria. Metabolomic profiling combined with a chemometric approach highlighted the variation of the antidiabetic properties of P. lentiscus according to the plant’s part and origin. A multiblock OPLS analysis showed that the metabolites most involved in α-glucosidase inhibition activity were mainly found in the stem bark extracts. The highest inhibitory activity was found for the stem bark extracts, with averaged inhibition percentage values of 84.7% and 69.9% for the harvested samples from the littoral and mountain, respectively. On the other hand, the fruit extracts showed a lower effect (13.6%) at both locations. The UHPLC-ESI-HRMS characterization of the metabolites most likely responsible for the α-glucosidase-inhibitory activity allowed the identification of six compounds: epigallocatechin(4a>8)epigallocatechin (two isomers), (epi)gallocatechin-3′-O-galloyl-(epi)gallocatechin (two isomers), 3,5-O-digalloylquinic acid and dihydroxy benzoic acid pentoside. Keywords: P. lentiscus, α-glucosidase, phytochemical profiling, UHPLC-ESI-HRMS, metabolomic approach, antidiabetic

Author Biographies

Chabha Sehaki, BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Laboratory of Natural Resources, University Mouloud Mammeri of Tizi-Ouzou, Tizi-Ouzou 15000, Algeria

Roland Molinie, BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Conceptualization, Methodology, Software, Validation, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review & editing, Visualization, Supervision

David Mathiron, Analytical Platform, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Conceptualization, Methodology, Software, Validation, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review & editing, Visualization, Supervision

Jean-Xavier Fontaine, BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Conceptualization, Methodology, Software, Validation, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review & editing, Visualization

Nathalie Jullian, BIOPI-UPJV Laboratory UMRT BioEcoAgro INRAE1158, SFR Condorcet FR CNRS 3417, UFR of Sciences, University of Picardie Jules Verne, 33 Rue Saint Leu, 80000 Amiens, France

Conceptualization, Validation, Writing – review & editing, Supervision, Project administration

Fadila Ayati, Laboratory of Natural Resources, University Mouloud Mammeri of Tizi-Ouzou, Tizi-Ouzou 15000, Algeria

Conceptualization, Writing – review & editing, Supervision

Farida Fernane, Laboratory of Natural Resources, University Mouloud Mammeri of Tizi-Ouzou, Tizi-Ouzou 15000, Algeria

Conceptualization, Validation, Investigation, Resources, Writing – review & editing, Visualization, Supervision, Project administration, Funding acquisition

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