Pharmacologically active herbal remedies against atherosclerosis, characterization and DoE based marker quantification by densitometry, and cell based assays on THP-1 cell lines
Authors
Rayadurgam Sireesha
aDepartment of Pharmaceutical Analysis, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Manikandan Krishnan
aDepartment of Pharmaceutical Analysis, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Muhammad Sadiq
bMolecular Biology and Immunobiology Division, Interdisciplinary Institute of Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Munuswamy Ganesh
bMolecular Biology and Immunobiology Division, Interdisciplinary Institute of Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Chinnaperumal Kamaraj
dInterdisciplinary Institute of Indian System of Medicine (IIISM), Directorate of Research, SRM Institute of Science and Technology (SRMIST), Kattankulathur 603203, Tamil Nadu, India
Naiyf Alharbi
eDepartment of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia
Muthu Thiruvengadam
fDepartment of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul 05029, Korea
Atherosclerosis is a complex condition that develops at varying rates in multiple configurations and blood vessels. The primary cause of morbidity and mortality worldwide, particularly in the industrialized nations, continues to be atherosclerosis. Ayurveda, Siddha, and Unani systems of medicine, among other traditional medical systems, utilize polyherbal compositions. The treatment of atherosclerosis has been improved with a novel multibotanical combination. In this study, we sought to formulate, characterize, and standardize a polyherbal formulation based on design of experiments (DoE), densitometric studies and to predict for antioxidant activity using molecular docking analysis based on LC- MS identified phytomarkers. In addition we have assessed its cell viability by MTT assay along with Ao/EtBr staining technique and intracellular ROS assay using THP-1 cell lines. Reported findings showed that the HPTLC based quantified components of selected multiherbals has the ability to treat for atherosclerosis. This document could be used to quickly authenticate the formulation as the method optimized was based on CCD design which shows desirability of 0.962 and 0.839. Cell based assays scientifically proves that the formulation was not toxic based on MTT assay along with AO/EtBr staining technique and has excellent antioxidant activities based on intracellular ROS assay using THP-1 cell lines. The observed findings would be crucial for future clinical aspects since the bioactive molecules contained in the extracts may have anticipated effects with other compounds and show a superior therapeutic potential. As a result, this study offers standardized and potentially therapeutic information about effective polyherbal formulation for atherosclerosis.
Keywords: Multibotanicals, HPTLC, AO/EtBr staining technique, MTT assay, Molecular docking, Intracellular ROS assay, Atherosclerosis
Author Biographies
Muhammad Sadiq, bMolecular Biology and Immunobiology Division, Interdisciplinary Institute of Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
cDepartment of Chemistry, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Munuswamy Ganesh, bMolecular Biology and Immunobiology Division, Interdisciplinary Institute of Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
cDepartment of Chemistry, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, India
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
