Preprint / Version 1

HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of Globularia alypum (L.), Infusion Extract

Authors

  • Sahar Nouir Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]
  • Bouraoui Ouni Laboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, Tunisia; [email protected] (R.S.); [email protected] (B.O.)
  • Khaled Saguem Laboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected] (A.L.); [email protected] (K.S.)
  • Yosra Hasni Department of Endocrinology, Farhat Hached Hospital of Sousse, Sousse 4000, Tunisia; [email protected]
  • Rim Bouhajeb Department of Pharmacology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]
  • Raoudha Slim Laboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, Tunisia; [email protected] (R.S.); [email protected] (B.O.)
  • Khaireddine Bouassida Urology Department, Sahloul Hospital, Sousse 4054, Tunisia; [email protected] (A.L.); [email protected] (K.B.)
  • Ahmed Loghmari Urology Department, Sahloul Hospital, Sousse 4054, Tunisia; [email protected] (A.L.); [email protected] (K.B.)
  • Aicha Laouani Laboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected] (A.L.); [email protected] (K.S.)
  • Amira Zaïri Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]

Keywords:

Globularia alypum L., HPLC-DAD, antioxidant activity, anti-arthritic activity, antiproliferative effect, antibacterial and antibiofilm activities

Abstract

Globularia alypum L. (GA) belonging to the Globulariaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, anti-arthritic, antiproliferative, antibacterial and antibiofilm potential of aqueous GA leaf extracts (AGAL). Quantitative analyses of the different constituents of extracts were evaluated by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). Spectrophotometric methods and chemical tests were used for antioxidant and anti-arthritic activities. The antiproliferative study was evaluated using colorectal cancer SW620 cells, while the antibacterial assessment and analysis of the antibiofilm effects were determined by the microdilution method and the crystal violet assay, respectively. AGAL extracts presented several components, mainly Nepetin-7-Glucoside and trans-ferrulic acid. The results showed that they had an important antioxidant (IC50 = 0.34; 0.38 and 1.20 mg/mL) and anti-arthritic (IC50 = 2.94 mg/mL) properties, and these effects are displayed in a dose-dependent manner. In addition, this extract demonstrated significant antiproliferative (IC50 = 50 µg/mL), antibacterial (MIC = 6.25 mg/mL and MBC = 6.25 mg/mL), and antibiofilm (59.70% at 25 mg/mL) properties especially against S. aureus. The results achieved confirm the important role of this plant as a source of therapeutic activities. Keywords: Globularia alypum L., HPLC-DAD, antioxidant activity, anti-arthritic activity, antiproliferative effect, antibacterial and antibiofilm activities

Author Biographies

Sahar Nouir, Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]

Methodology

Bouraoui Ouni, Laboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, Tunisia; [email protected] (R.S.); [email protected] (B.O.)

Investigation

Khaled Saguem, Laboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected] (A.L.); [email protected] (K.S.)

Methodology, Funding acquisition

Yosra Hasni, Department of Endocrinology, Farhat Hached Hospital of Sousse, Sousse 4000, Tunisia; [email protected]

Investigation

Rim Bouhajeb, Department of Pharmacology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]

Methodology

Raoudha Slim, Laboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, Tunisia; [email protected] (R.S.); [email protected] (B.O.)

Investigation, Funding acquisition

Khaireddine Bouassida, Urology Department, Sahloul Hospital, Sousse 4054, Tunisia; [email protected] (A.L.); [email protected] (K.B.)

Software, Resources

Ahmed Loghmari, Urology Department, Sahloul Hospital, Sousse 4054, Tunisia; [email protected] (A.L.); [email protected] (K.B.)

Software, Resources

Aicha Laouani, Laboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected] (A.L.); [email protected] (K.S.)

Methodology

Amira Zaïri, Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia; [email protected]

Methodology, Investigation, Writing – original draft

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