Preprint / Version 1

Pharmaceutical characterization and exploration of Arkeshwara rasa in MDA-MB-231 cells

Authors

  • Remya Jayakumar aDepartment of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India
  • SK Trigun dDepartment of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India
  • Zeeshan Fatima gDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, 61922, Saudi Arabia
  • Kaushavi Cholke eAmity Lipidomics Research Facility (ALRF), Amity University, Haryana, Manesar, Gurugram, 122413, India
  • Akanksha Pandey dDepartment of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India
  • Saumya Gulati cDept of Rasashastra and Bhaishjya Kalpana, Babu Yugraj Singh Ayurvedic Medical College and Hospital, Gomtinagar Extension, Sector 6 Lucknow, Uttar Pradesh, 226010, India
  • Shiwakshi Sharma aDepartment of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India
  • Pankaj Kumar aDepartment of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India
  • Manoj Dash bDepartment of Rasashastra and Bhaishajya Kalpana, Government Ayurveda College, Raipur, India
  • Namrata Joshi aDepartment of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India

Keywords:

Traditional medicine, Cancer, IC50 value, Hesperidin, In-vitro, Arkeshwara rasa

Abstract

The diverse specificity mode of cancer treatment targets and chemo resistance demands the necessity of drug entities which can address the devastating dynamicity of the disease. Objectives To check the anti-tumour potential of traditional medicine rich in polyherbal components and metal nanoparticle namely Arkeshwara rasa (AR). Material methods The AR was prepared in a modified version with reference from Rasaratna Samuchaya and characterized using sophisticated instrumental analysis including XRD, SEM-EDAX, TEM, TGA-DSC, and LC-MS and tested against the MDA-MB-231 cell line to screen cell viability and the cytotoxicity with MTT, SRB and the AO assay. Results XRD pattern shows cubic tetrahedrite structure with Sb, Cu, S peaks and trace elements like Fe, Mg, etc. The particle size of AR ranges between 20 and 30 nm. The TGA points thermal decomposition at 210 °C and the metal sulphide peaks in DSC. LC-MS analysis reveals the components of the formulation more on the flavonoid portion. The IC50 value of MTT and SRB are 25.28 μg/mL and 31.7 μg/mL respectively. The AO colorimeter substantiated the cell viability and the apoptosis figures of the same cell line. The AR exhibits cytotoxicity and reaffirms the apoptosis fraction with SRB assay. Conclusions The Hesperidine, Neohesperidin, Rutin components in the phytochemical pool can synergize the anti-tumour potential with either influencing cellular pathways or decreasing chemo resistance to conventional treatment. AR need to be further experimented with reverse transcription, flow cytometry, western blotting, etc. Keywords: Traditional medicine, Cancer, IC50 value, Hesperidin, In-vitro, Arkeshwara rasa

Author Biographies

Zeeshan Fatima, gDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, 61922, Saudi Arabia

hAmity Institute of Biotechnology, Amity University, Haryana, Manesar, Gurugram, 122413, India

Kaushavi Cholke, eAmity Lipidomics Research Facility (ALRF), Amity University, Haryana, Manesar, Gurugram, 122413, India

fInstitute of Biochemistry and Molecular Medicine, University of Bern, 3012, Switzerland

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