Preprint / Version 1

Iridoid Glycosides and Coumarin Glycoside Derivatives from the Roots of Nymphoides peltata and Their In Vitro Wound Healing Properties

Authors

  • Tae-Young Kim Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);
  • Bum Lee School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; [email protected]
  • Beom-Geun Jo Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);
  • Seong Heo Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea; [email protected] (S.P.H.); [email protected] (S.-N.K.)
  • Young Jung Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);
  • Su-Nam Kim Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea; [email protected] (S.P.H.); [email protected] (S.-N.K.)
  • Ki Kim School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; [email protected]
  • Min Yang Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);

Keywords:

Nymphoides peltata, peltatamarin A, peltatamarin B, human keratinocyte cell, wound healing, cell migration, cell proliferation

Abstract

Nymphoides peltata has been used as a medicinal herb in traditional medicines to treat strangury, polyuria, and swelling. The phytochemical investigation of the MeOH extract of N. peltata roots led to the isolation of three iridoid glycosides and three coumarin glycoside derivatives, which were characterized as menthiafolin (1), threoninosecologanin (2), callicoside C (3), and scopolin (4), as well as two undescribed peltatamarins A (5) and B (6). The chemical structures of the undescribed compounds were determined by analyzing their 1 dimensional (D) and 2D nuclear magnetic resonance (NMR) spectra and using high-resolution (HR)-electrospray ionization mass spectroscopy (ESI-MS), along with the chemical reaction of acid hydrolysis. The wound healing activities of the isolated compounds 1–6 were evaluated using a HaCaT cell scratch test. Among the isolates, scopolin (4) and peltatamarin A (5) promoted HaCaT cell migration over scratch wounds, and compound 5 was the most effective. Furthermore, compound 5 significantly promoted cell migration without adversely affecting cell proliferation, even when treated at a high dose (100 μM). Our results demonstrate that peltatamarin A (5), isolated from N. peltata roots, has the potential for wound healing effects. Keywords: Nymphoides peltata, peltatamarin A, peltatamarin B, human keratinocyte cell, wound healing, cell migration, cell proliferation

Author Biographies

Tae-Young Kim, Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);

Investigation

Bum Lee, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; [email protected]

Investigation

Beom-Geun Jo, Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);

Formal analysis

Seong Heo, Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea; [email protected] (S.P.H.); [email protected] (S.-N.K.)

Formal analysis

Young Jung, Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);

Formal analysis

Su-Nam Kim, Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea; [email protected] (S.P.H.); [email protected] (S.-N.K.)

Conceptualization

Ki Kim, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; [email protected]

Conceptualization

Min Yang, Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea; [email protected] (T.-Y.K.); [email protected] (B.-G.J.);

Conceptualization

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