Evaluation and comparison of antibacterial efficacy of different concentrations of Chhattisgarh herbal product—Terminalia chebula fruit extract in opposition to Enterococcus faecalis: An in vitro study
Authors
Shivani Doye
Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, Durg, Chhattisgarh, India
Manjunath Malur
Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, Durg, Chhattisgarh, India
Yogesh Sahu
Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, Durg, Chhattisgarh, India
Ankita Singh
Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, Durg, Chhattisgarh, India
Praveen Mishra
Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, Durg, Chhattisgarh, India
Roshan Mohamed
Department of Pediatric Dentistry, Faculty of Dentistry, Taif University, Taif, Saudi Arabia
Mohmed Karobari
Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Puthisastra, Phnom Penh, Cambodia
Due to the low cost, natural origin, higher safety margins, and little to negligible adverse effects of herbal medications, the use of plants and plant derivatives in medicine is becoming increasingly widespread. Terminalia chebula is among the most significant medicinal plants in ayurvedic, siddha, unani, and homeopathic remedies. It is ranked first in Ayurvedic material medicine. T. chebula has been shown to have established effects against various bacterial and fungal infections, including dental caries pathogens. In recent years, there has been a rise in interest in dentistry and medicine related to Enterococcus faecalis. The research aimed to assess the antibacterial effectiveness of different concentrations of T. chebula ethanolic fruit extract (10%, 40%, and 100%) in opposition to E. faecalis and compare it with 2% chlorhexidine. For the study, T. chebula ethanolic fruit extracts were obtained and prepared with Group I: −10% concentration, Group II: −40% concentration, Group III: −100% concentration, and Group IV: −2% chlorhexidine. Colonies of E. faecalis were cultivated in brain heart infusion (BHI) broth at 37°C and were inoculated in 16 BHI agar plates. Then, on the petri dishes, four wells were created (8 mm diameter) using a metal borer. The Agar well diffusion method was used to examine the antibacterial activity, and the zones of inhibition around the wells were noted. The obtained data were statistically analyzed using one‐way ANOVA and post‐hoc tests. The result shows that as the concentration increases, there is an increase in the efficacy of the antibacterial property of the extract before it reaches the saturation point. The decreasing order of antibacterial was chlorhexidine >100% T. chebula >40% T. chebula >10% T. chebula. The production of contemporary pharmaceuticals from T. chebula was addressed, as the global scenario is currently evolving toward using nontoxic plant products with traditional medicinal benefits.
Keywords: antibacterial activity, dental, endodontics, Enterococcus faecalis, herbal product, Terminalia chebula extract
Author Biography
Mohmed Karobari, Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Puthisastra, Phnom Penh, Cambodia
Dental Research Unit, Center for Global Health Research, Saveetha Institute of Medical and Technical Sciences University, Chennai, Tamil Nadu, India
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
