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Cycloolivil Isolated from Nardostachys jatamansi Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes

Authors

Keywords:

Nardostachys jatamansi, anti-skin inflammation, keratinocytes, JAK/STAT, NF-κB

Abstract

Nardostachys jatamansi is widely used as a traditional medicine in Asian countries. Numerous recent studies have reported the biological activities of its secondary metabolites and extracts. In this study, a total of 14 components were isolated, including cycloolivil and 2-(3′-hydroxy-5′-ethoxyphenyl)-3-hydroxylmethyl-7-methoxy-2,3-dihydrobenzofuran-5-carboxylic acid, which were first discovered in N. jatamansi. The isolated compounds were investigated for their anti-inflammatory effects on HaCaT keratinocytes and their potential to alleviate skin inflammation. The results of the screening revealed that cycloolivil and 4β-hydroxy-8β-methoxy-10-methylene-2,9-dioxatricyclo[4.3.1.03,7]decane reduced the production of inflammatory cytokines induced by TNF-α/IFN-γ, such as IL-6, IL-8, and RANTES, in keratinocytes. This study focused on exploring the biological effects of cycloolivil, and the results suggested that cycloolivil inhibits the expression of COX-2 proteins. Further mechanistic evaluations confirmed that the anti-inflammatory effects of cycloolivil were mediated by blockage of the NF-κB and JAK/STAT signaling pathways. These results suggest that cycloolivil isolated from N. jatamansi could be used to treat skin inflammatory diseases. Keywords: Nardostachys jatamansi, anti-skin inflammation, keratinocytes, JAK/STAT, NF-κB

Author Biographies

Chi-Su Yoon, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of Korea; [email protected]

Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review & editing, Visualization

Hwan Lee, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review & editing, Visualization

Zhiming Liu, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Investigation, Data curation

Linsha Dong, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Investigation, Data curation

Gyoyoung Lee, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Investigation, Data curation

Nayeon Kim, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Investigation, Data curation

Hyuncheol Oh, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of Korea; [email protected]

Resources, Writing – review & editing, Supervision, Project administration, Funding acquisition

Dong-Sung Lee, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea; [email protected] (H.L.); [email protected] (Z.L.); [email protected] (L.D.); [email protected] (G.L.); [email protected] (N.K.)

Conceptualization, Writing – original draft, Writing – review & editing, Supervision, Project administration, Funding acquisition

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