Preprint / Version 1

Phytochemical profiling of the stem bark of Betula utilis from different geographical regions of India using UHPLC‐ESI‐MS/MS

Authors

  • Tripti Mishra Phytochemistry Division, CSIR‐National Botanical Research Institute, Lucknow, India
  • Preeti Chandra Sophisticated Analytical Instrument Facility, CSIR‐Central Drug Research Institute, Lucknow, India
  • Brijesh Kumar Sophisticated Analytical Instrument Facility, CSIR‐Central Drug Research Institute, Lucknow, India
  • Meena Baleshwar Plant Diversity, Systematics and Herbarium Division, CSIR‐National Botanical Research Institute, Lucknow, India
  • Pushpa Joshi Department of Chemistry, DSB Campus, Kumaun University, Nainital, India
  • Tikam Rana Plant Diversity, Systematics and Herbarium Division, CSIR‐National Botanical Research Institute, Lucknow, India
  • Dalip Upreti Plant Diversity, Systematics and Herbarium Division, CSIR‐National Botanical Research Institute, Lucknow, India
  • Mahesh Pal Phytochemistry Division, CSIR‐National Botanical Research Institute, Lucknow, India

Keywords:

Betula utilis, quantitation, tandem mass spectrometry, triterpenoids, UHPLC

Abstract

A simple, rapid, sensitive, and reliable ultra‐high‐performance liquid chromatography‐hybrid linear ion trap triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 10 bioactive compounds in stem bark of Betula utilis grown in high altitude of Himalaya, India. The objective of the study is to develop and validate ultra‐high‐performance liquid chromatography‐hybrid linear ion trap triple quadrupole mass spectrometry for investigation of geographical variations of triterpenoids, phenolics, and flavonoids contents in stem bark of B. utilis. The validated method was successfully applied to investigate geographical variations of triterpenoids, phenolics, and flavonoids in stem bark of B. utilis. The contents of betulinic acid and oleanolic acid were detected higher among selected analytes. The present variation study reveals great importance for the application and overall assessment of B. utilis. Keywords: Betula utilis, quantitation, tandem mass spectrometry, triterpenoids, UHPLC

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