Phenolic enriched fraction of Clerodendrum glandulosum Lindl. leaf extract ameliorates hyperglycemia and oxidative stress in streptozotocin-nicotinamide induced diabetic rats
Authors
Puspanjali Khound
aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
Prashanta Deb
aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
Swarnali Bhattacharjee
aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
Karla Medina
dCenter for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Food Technology Unit, 45019, Jalisco, Mexico
Partha Sarma
aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
Biswatrish Sarkar
cDepartment of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Jharkhand, 835215, India
Rajlakshmi Devi
aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
Clerodendrum glandulosum Lindl. is an important ethnomedicinal shrub of Northeast India, used by traditional healers to control various ailments like diabetes, hypertension, arthritis, etc.
Objectives
The present study was conducted to explore the anti-hyperglycemic and antioxidative effects of the polyphenol-rich fraction (PRF) of C. glandulosum leaf extract and identification of its major bioactive compounds. Further, an in-silico molecular docking study was also performed to understand the molecular interactions of the identified major compounds with some target proteins associated with diabetic complications.
Materials and methods
PRF was purified from the hydromethanolic (80% MeOH) extract of leaves and subjected to assessment of in-vitro antioxidant and anti-diabetic properties. It was also subjected to evaluate the ameliorative effect during streptozotocin-nicotinamide-induced hyperglycemia in Wistar albino rats. An in-silico molecular docking study was also performed to complement the in-vitro/in-vivo studies.
Results
Chemical analysis of PRF showed the presence of phenolics like caffeic acid, verbascoside, isoverbascoside, and apigenin, of which verbascoside (598.14 ± 1.24 mg/g) was found to be the principal compound. In-vitro studies showed potent antioxidant (IC50 of DPPH:32.45 ± 2.16 μg/mL; ABTS:39.08 ± 0.53 μg/mL) properties and excellent aldose reductase inhibition potential (IC50 2.18 ± 0.10 μg/mL). Treatment with PRF showed reduced blood glucose levels and increased plasma insulin levels. The results also indicate an improvement of endogenous antioxidants and suppression of inflammatory cytokines (IL-6 and TNF-α) comparable to the standard. Molecular docking studies predicted promising interactions between the identified molecules and the crucial amino acid residues of the enzymes involved in the development of hyperglycemia.
Conclusion
This study revealed the antihyperglycemic and antioxidant potential of partially purified fraction PRF of C. glandulosum leaves.
Keywords: Antioxidant, Clerodendrum glandulosum Lindl., Hyperglycemia, Oxidative stress, Phenolic compounds, Diabetes
Author Biographies
Puspanjali Khound, aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
bDepartment of Zoology, Gauhati University, Jalukbari, Guwahati, Assam, 781014, India
Prashanta Deb, aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
cDepartment of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Jharkhand, 835215, India
Swarnali Bhattacharjee, aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
bDepartment of Zoology, Gauhati University, Jalukbari, Guwahati, Assam, 781014, India
Rajlakshmi Devi, aLife Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, 781035, India
bDepartment of Zoology, Gauhati University, Jalukbari, Guwahati, Assam, 781014, India
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