Insights into the potential dual-antibacterial mechanism of Kelisha capsule on Escherichia coli
Authors
Guolin Shi
Department of Ultrasound in Medicine, School of Medicine, The Second Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Shangcheng District, Hangzhou, 310009 China
Xiao Lu
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Yuanhang Zheng
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Tao Yang
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Enyuan Zhu
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Yanhong Song
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Pintong Huang
Department of Ultrasound in Medicine, School of Medicine, The Second Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Shangcheng District, Hangzhou, 310009 China
Traditional Chinese medicine (TCM), AYURVEDA and Indian medicine are essential in disease prevention and treatment. Kelisha capsule (KLSC), a TCM formula listed in the Chinese Pharmacopoeia, has been clinically proven to possess potent antibacterial properties. However, the precise antimicrobial mechanism of KLSC remained unknown. This study aimed to elucidate the dual antibacterial mechanism of KLSC using network pharmacology, molecular docking, and experimental validation. By analyzing the growth curve of Escherichia coli (E. coli), it was observed that KLSC significantly inhibited its growth, showcasing a remarkable antibacterial effect. Furthermore, SEM and TEM analysis revealed that KLSC damaged the cell wall and membrane of E. coli, resulting in cytoplasmic leakage, bacterial death, and the exertion of antibacterial effects. The network pharmacology analysis revealed that KLSC exhibited an effect on E. coli ATP synthase, thereby influencing the energy metabolism process. The molecular docking outcomes provided evidence that the active compounds of KLSC could effectively bind to the ATP synthase subunit. Subsequently, experimental findings substantiated that KLSC effectively suppressed the activity of ATP synthase in E. coli and consequently decreased the ATP content. This study highlighted the dual antibacterial mechanism of KLSC, emphasizing its effects on cell structure and energy metabolism, suggesting its potential as a natural antibacterial agent for E. coli-related infections. These findings offered new insights into exploring the antibacterial mechanisms of TCM by focusing on the energy metabolism process.
Keywords: Kelisha capsule, Natural medicine, Antibacterial mechanism, Network pharmacology, Molecular docking, ATP synthase
Author Biographies
Guolin Shi, Department of Ultrasound in Medicine, School of Medicine, The Second Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Shangcheng District, Hangzhou, 310009 China
Post-Doctoral Research Center, Zhejiang SUKEAN Pharmaceutical Co., Ltd, Hangzhou, 311228 China
Pintong Huang, Department of Ultrasound in Medicine, School of Medicine, The Second Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Shangcheng District, Hangzhou, 310009 China
Research Center for Life Science and Human Health, Binjiang Institute of Zhejiang University, Hangzhou, 310053 China
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