Preprint / Version 1

Screening for anti-influenza virus compounds from traditional Mongolian medicine by GFP-based reporter virus

Authors

  • Mao-Shun Nie College of Veterinary Medicine, Yangzhou University, Yangzhou, China
  • Xiao-He Li College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China
  • Sen Zhang State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
  • Dan-Dan Zeng State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
  • Yu-Rong Cai State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
  • Da-Xin Peng College of Veterinary Medicine, Yangzhou University, Yangzhou, China
  • Tao Jiang State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
  • Jian-Ping Shi College of Traditional Chinese Medicine, Inner Mongolia Medical University, Hohhot, China
  • Jing Li College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China

Keywords:

GFP-based reporter virus, influenza A virus, traditional mongolian medicine, kaempferide, Curcumin, Cardamonin

Abstract

Introduction Screening for effective antiviral compounds from traditional Mongolian medicine not only aids in the research of antiviral mechanisms of traditional medicines, but is also of significant importance for the development of new antiviral drugs targeting influenza A virus. Our study aimed to establish high-throughput, rapid screening methods for antiviral compounds against influenza A virus from abundant resources of Mongolian medicine. Methods The use of GFP-based reporter viruses plays a pivotal role in antiviral drugs screening by enabling rapid and precise identification of compounds that inhibit viral replication. Herein, a GFP-based reporter influenza A virus was used to identify potent anti-influenza compounds within traditional Mongolian medicine. Results Our study led to the discovery of three active compounds: Cardamonin, Curcumin, and Kaempferide, all of which exhibited significant antiviral properties in vitro. Subsequent analysis confirmed that their effectiveness was largely due to the stimulation of the antiviral signaling pathways of host cells, rather than direct interference with the viral components, such as the viral polymerase. Discussion This study showcased the use of GFP-based reporter viruses in high-throughput screening to unearth antiviral agents from traditional Mongolian medicine, which contains rich antiviral compounds and deserves further exploration. Despite certain limitations, fluorescent reporter viruses present substantial potential for antiviral drug screening research due to their high throughput and efficiency. Keywords: GFP-based reporter virus, influenza A virus, traditional mongolian medicine, kaempferide, Curcumin, Cardamonin

Author Biography

Jing Li, College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China

State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China

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