Preprint / Version 1

Evaluation of anticancer potential of Thai medicinal herb extracts against cholangiocarcinoma cell lines

Authors

  • Bundit Promraksa
  • Anchalee Techasen
  • Investigation Investigation
  • Methodology Methodology
  • Resources Resources
  • Supervision Supervision
  • Writing editing Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Patcharee Boonsiri
  • Resources Resources
  • Writing editing Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Watcharin Loilome
  • Conceptualization Conceptualization
  • Data curation
  • Formal analysis
  • Funding acquisition
  • Investigation Investigation
  • Methodology Methodology
  • Project administration
  • Writing editing Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Supervision Supervision
  • Conceptualization Conceptualization
  • Data curation
  • Formal analysis
  • Investigation Investigation
  • Methodology Methodology
  • Validation Validation
  • Visualization Visualization
  • Writing draft Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Jutarop Phetcharaburanin
  • Data curation
  • Formal analysis
  • Investigation Investigation
  • Methodology Methodology
  • Writing editing Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Nisana Namwat
  • Investigation Investigation
  • Methodology Methodology
  • Supervision Supervision

Keywords:

None

Abstract

Although cholangiocarcinoma (CCA) has a low incidence globally, this is extremely high in Northeast Thailand. The lack of both early detection measures and effective therapeutic drugs is the major problem for the poor prognosis of CCA patients. Based on regional knowledge, it would be advantageous to search for effective natural phyto-products for the treatment of CCA. Cardiospermum halicacabum L., Gomphrena celosioides Mart. and Scoparia dulcis L., very well-known medicinal herbs in Asian countries, were selected for the investigation of inhibitory effects on CCA cells. Of the three different ethanolic extracts, S. dulcis L extract showed most inhibitory effects on cell growth of CCA cell lines KKU-100 and KKU-213, at percentages of 56.06 and 74.76, respectively, compared to the untreated group after treatment with 250 μg/mL of extracts for 72 hrs. At 400 and 500 μg/mL of the extracts, the inhibitory effect of KKU-213 was indicated by a significant increase in the BAX/Bcl-2 ratio and cell membrane permeability. Moreover, metabolic profiling-based screening employed in the current study revealed a significant positive association between the lignin compound and a decrease in CCA cell viability. Our study suggests, for the first time, that ESD has the ability to inhibit CCA cell growth through the induction of apoptosis.

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Author Biographies

Writing editing, Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen, Thailand

Writing editing, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Writing draft, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Writing editing, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Center of Excellence for Innovation in Chemistry, Mahidol University, Bangkok, Thailand

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Posted

2024-08-01